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Sue Ann bound the reaper to have it do her bidding. She targets people she considers immoral in order to save “good” people.
Layla—young woman with a brain tumor. Her mother is aggressively protective of her and dislikes Dean for prolonging Layla’s recovery.
Sam and Dean fail to save Layla.
Gets electrocuted during a hunt which results in a heart attack and certain death within a few weeks. Sam has to save his life via the faith healer.
Sue Ann targets Dean after he meddles in her work, and Sam has to save him again.
Cyrus Dorian’s soul fused with his truck and manifests to kill the people who were involved in his murder after the mayor tears down his house, disrupting his spirit.
Cassie—Dean’s old girlfriend from while Sam was in college. Dean told her about hunting and she broke up with him, thinking he was crazy.
Dean gets in a car chase with the racist truck and Sam gives Dean directions to the location of the church Cyrus burned down to destRoy the spirit
One of Azazel’s Special Children; he has the power of telekinesis. He’s taking revenge on his family for abusing him.
Alice—Max’s step-mother, who is victimized by Max for allowing her husband and brother-in-law to abuse him.
Despite Max not wanting to kill Dean originally, Dean tries to protect Alice by putting himself in front of him, which causes Max to target him first. Sam has to save him using his psychic powers.
Cannibalistic humans who hunt people for sport. They choose two humans per year.
Kathleen—deputy in Hibbing who lost her brother three years prior to the Benders’ human hunts. She wants to arrest Dean for impersonating an officer.
Sam is captured by the Benders and Dean has to rescue him.
Meg is luring Sam and Dean into a trap in order to draw out John. She uses a Daeva to murder people from Lawrence to catch their attention.
The Daevas are not actually killed, only stopped temporarily.
None
They both get captured by Meg, but through both of their diversions Sam is able to break free and destRoy her altar and turn the Daevas on her.
As part of a prank, some teenagers draw a tibetan symbol on an old abandoned house which summons a tulpa. The tulpa itself has no motivation outside of what people believe it does.
Ed Zeddmore and Harry Spengler (Ghostfacers)—meddlesome ghost hunters who keep getting in Sam and Dean’s way.
Harry is reluctant to hunt ghosts but follows along out of a sense of cameraderie.
A centuries-old witch who feeds on the spiritus vitae (souls) of children to stay alive. Any souls will do, but children’s souls are heartier.
15-20 year feeding cycle (previous cycle was 16-17 years ago; 1989-1990).
Michael—the older brother of one of the Shtriga’s victims, he is used as bait to lure out the Shtriga.
During the last feeding cycle, Sam was victimized by the Shtriga because Dean left him alone at their motel. He almost died from the attack, but John saved him, and since then Dean has blamed himself for his failure to protect Sam.
Young girl who murdered her family and then murdered her adoptive family as well. Her soul is trapped in a painting and she kills the families who buy it.
Sam and Dean originally think the murderer is Isaiah Merchant, Melanie’s adopted father, because he looks like a killer.
Sarah—antique dealer who develops a crush on Sam and wants to stop the killings because she feels responsible for them.
Sam and Sarah get trapped inside of a house with the ghost, and Dean has to find her remains and burn them to save them.
A vampire nest who has killed a hunter (Daniel Elkins) out of revenge for the leader of the nest (Luther). They mostly keep a low profile because they’re at risk of extinction, but Elkins’s death puts Sam and Dean on their trail. Vampires were previously thought to be extinct (John believed Elkins killed the last one), and Dean thought they were entirely fictional.
Jenny—a victim of the nest. She’s turned into a vampire and becomes loyal to them.
Various other victims.
Dean is used as bait to lure out Kate. She sexually assaults him, and Sam and John attack her with arrows coated in dead man’s blood.
Luther grabs Sam and uses him as a hostage to make Dean stop attacking them. John kills him with the Colt.
The Winchester family = vampire nest; John = Luther; Dean = Kate; Sam = Jenny
Luther = John: they both know about the Colt while the others in their families are ignorant of it; they are the leader and patriarch of the nest; and they both sacrifice themselves for their families’ safety (see 1x03 Dead in the Water and 2x01 In My Time of Dying)
John admits that hunting Azazel is a suicide mission. he has been trying to keep Sam and Dean away because he doesn’t want them to see him get hurt or die, and he doesn’t want to see them potentially die either: “i can’t watch my children die, too.” compare 2x01 In My Time of Dying, where he sacrifices himself to Azazel for Dean’s life. he was destined to die on this hunt, and he accepted that long ago which is why he can die for Dean so easily
Dean “we’re stronger as a family”
luther grabs Sam and uses him as a hostage to threaten Dean. it’s a direct parallel to John using kate as his hostage. again the vampires are paralleled with the Winchesters and proves their love makes them weak. Dean can’t let Sam get hurt, so he puts down his weapons just like luther did to protect kate. luther’s love for kate is romantic, which implies lovers and family are one and the Same
John “we are stronger as a family”
Meg is murdering John’s hunter friends to coerce him into giving her the Colt.
Azazel is revealed to be creating Special Children again and has been since about the time Sam started having visions pre-season 1.
Several of John’s hunting friends—Pastor Jim Murphy and Caleb are the two Meg manages to kill, and her next victim is unknown.
Monica and baby Rose—Rose is supposed to be one of Azazel’s special children, but Sam thwarts Azazel’s attempt to feed her blood. Their house is still set on fire, though, and Sam and Dean rescue the whole family.
Meg has captured John and given him to Azazel to possess, and together they lure Sam and Dean into a trap. Meg is exorcized early into the episode.
John—he’s beaten by Meg and her brother (also a demon) and they give him to Azazel, who possesses him.
Azazel pins Dean to a wall to coax Sam to use his powers, nearly killing him as he tortures him to tease Sam.
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